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Fig. 2
Low temperature acclimation helps to maintain the ZF4 cell cytoskeleton. F-actin was labeled with rhodamin-phalloidin following exposure to low temperatures at different time points in ZF4 cells. The cell cytoskeletons were stained with phalloidin (red) and the staining of nuclei was performed with DAPI (blue). Laser scanning confocal microscopy was used to visualize the cytoskeleton of the cell. Scale bar = 25 μm. Ctr, the control group; Dg, the domesticated group; Ng, the non-domesticated group.
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Full text @ Front. Physiol.