ZFIN Image: Wang et al., 2022, Fig. 1

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Figure Caption

Fig. 1

Effect of cold stress on cell morphology and cell viability. Cells were allowed to grow in the incubator at 28°C in 5% CO2 and then treated with low temperature (13 and 18°C). Ctr, the control group; Dg, the domesticated group; Ng, the non-domesticated group. (A) Morphological pictures of ZF4 cells under normal or low temperature conditions. Cell morphology was examined under an inverted microscope at a 20 × magnification. Scale bar = 100 μm. (B,C) The CKK-8 assay was used to measure the viability of groups of short-term low temperature stress and long-term low temperature acclimation cells. The experiments were repeated three times. The results are presented as the mean ± SD (n = 6); *P < 0.05, **P < 0.01.

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