ZFIN Image: Blum et al., 2023, Fig. 2.

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Figure Caption

Fig. 2.

Long bone overgrowth is caused by PI3K/AKT overactivation in mesenchymal derivatives. PI3K/AKT mosaic overactivation results in an early overgrowth phenotype in the fin endoskeleton. (A,B) In vivo confocal imaging of chondrocytes (A) marked by Tg(sox10:DsRed) (magenta) and osteoblasts (B) marked by Tg(sp7:mCherry) (magenta) in late larval stages reveals localized skeletal overgrowth in median fins of akt1^E18K-GFP (green) and myr-p110-GFP (green) mosaic larva overlapping with GFP⁺ cells. Boxed areas are at high magnification, showing that akt1^E18K-GFP and myr-p110-GFP colocalize with chondrocytes and osteoblasts. Asterisks indicate overgrown bones. Arrowheads indicate GFP⁺ chondrocytes in A and GFP⁺ osteoblasts in B. SL, standard length in mm. Scale bars: 100 ?m; high-magnification images: 25 ?m.

Acknowledgments

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