ZFIN Image: Yang et al., 2023, Figure 1.

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Figure Caption

Figure 1.

Morroniside promotes the proliferation and differentiation of MC3T3-E1 cells. (A) Cell viability was measured after treatment with different concentrations of morroniside (10, 20, 40 and 80 μM). (B) After 20 μM of MOR treatment, cell viability was monitored over 72 h, and a time-dependent increase in cell viability was observed. (C) The expression of p21, Ccnd1 and PCNA was determined by RT-PCR. (D) The effects of MOR on MC3T3-E1 cell differentiation and alkaline phosphatase (ALP) activity as shown by alizarin red staining (ARS) and ALP staining. Quantitative analysis of ARS (E) and ALP stating (F). *p< 0.05, **p< 0.01 vs. Ctl. n = 4–6.

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