Figure 6

Effect of Psen2 absence on autophagy. (a,b) Representative blots and relative quantifications of LC3-II levels (normalized to tubulin levels) in WT and psen2^−/− embryos or larvae at (a) 2- and (b) 8-dpf, respectively, in basal conditions or upon the induction of autophagy (by rapamycin, RAPA), in the presence or not of the lysosomal inhibitor bafilomycin (BAF). (a) Pools of 15 WT and psen2^−/− embryos were treated with either vehicle (DMSO) or 1 µM Rapamycin (Rapa) from 8- to 48-hpf (to induce autophagy), with or without the addition of 40 nM Bafilomycin (Baf) at 32-hpf (final 16 h-treatment with the lysosomal inhibitor). Mean ± SEM, n = 3 independent experiments. (b) Pools of 10 6-dpf WT and psen2^−/− larvae either fed (3 times per day) or starved for 2 days, treated or not with 40 nM BAF for the last 16 h of the experiment. Mean ± SEM, n = 3 independent experiments. (c) Representative blot and relative quantification of LC3-II levels (normalized to tubulin levels) in the brains of psen2^+/+ and psen2^−/− 6-month-old fish. Data were generated from 3 brains for each genotype. Values represent the mean ± SEM. * = p < 0.05; ** = p < 0.01; *** = p < 0.001.