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Figure 4.
(A) Gigapixel video of slime mold (P. polycephalum) growth on maze (16.5 cm per side). Four mold samples were taken from a seed inoculation on another petri dish and placed in four corners of the maze. Recording for 96 hr shows the slime mold growth within the maze (Video 7). highlighting the MCAM’s ability to simultaneously observe the macroscopic maze structure while maintaining spatial and temporal resolution sufficient to observe microscopic cytoplasmic flow (imaging experiments with P. polycephalum repeated 5 times). (B) Gigapixel video of a small colony of Carpenter ants during light and dark alternations. Overall activity (velocity) of ants measured with optical flow increased during light off versus light on phases. Full MCAM frame observes Carpenter ant colony movement and clustering within large arena, while zoom-in demonstrates spatial resolution sufficient for leg movement and resolving hair on abdomen (imaging experiments with Carpenter ants repeated 2 times). See Video 10. (C) Adult Drosophila gigapixel video frame showing collective behavior of several dozen organisms at high spatial resolution, with insets revealing fine detail (e.g. wings, legs, eyes) during interaction (imaging experiments with adult Drosophila repeated 2 times). See Video 11.
MCAM imaging of behaviors in multiple small organisms.