ZFIN Image: Rashid et al., 2021, Fig. 7

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Figure Caption

Fig. 7

HI-NESS does not interfere with cell cycle progression. Panel A: U2OS (FRT) cells in the S-phase were visualised (Wide field microscopy, single Z-plane) by pulse-labelling the cells with EdU and ‘clicking’ the molecule to 5/6-FAM. Panel B: 56.6 ± 2.9% of U2OS (FRT) cells expressing mEGFP-HI-NESS (U2OS-HI-NESS) occur in the S-phase of the cell cycle compared to 65 ± 7.2% of cells expressing mEGFP-NLS (U2OS-mEGFP). Flow cytometry of propidium iodide-labelled cells (Panels C–E) detects 37% of U2OS-HI-NESS cells in the G1 phase of the cell cycle, 33% in the S phase and 30% in the G2/M phase (Panels C and E). Of the U2OS-mEGFP cells, 38% occur in the G1 phase, 32% in the S phase and 30% in the G2/M phase (Panels D and E).

Acknowledgments

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