ZFIN Image: Rashid et al., 2021, Fig. 6

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Fig. 6

HI-NESS does not induce increased rates of DNA damage. The induction of γH2AX—a DNA damage biomarker—in U2OS (FRT) cells stably expressing mEGFP-HI-NESS is comparable to cells expressing mEGFP-NLS as visualised by microscopy (Wide field microscopy, single Z-plane) (Panel A, left). On average, 7.4 ± 3.0 γH2AX foci were detected in U2OS cells expressing mEGFP-HI-NESS cells, compared to 9.3 ± 2.9 foci with mEGFP-NLS expression (Panel B, green). γH2AX is induced when the cells are treated with 3 Gy ionizing radiation (IR) (Panel A, right), 51.0 ± 6.4 γH2AX foci appear in U2OS (FRT) mEGFP-HI-NESS cells, and 57.9 ± 2.6 in U2OS (FRT) mEGFP-NLS cells (Panel B, magenta). Panel C: Western blot shows that γH2AX induction is unaffected by the expression of HI-NESS. Furthermore, γH2AX induction and hence, the DNA damage response, following IR treatment is not affected by HI-NESS.

Acknowledgments

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