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Figure 1
Analysis of tmem38a/b across species and of their spatio-temporal expression during zebrafish development. (A) The sinteny analysis performed on humans (H. sapiens), mice (M. musculus) and zebrafish (D. rerio) supported the existence of a common ancestral chromosomal origin for both tmem38a and tmem38b. The maps were obtained using the genome browser Genomicus. The human genes were used as roots. The position of the genes (Mb) relative to the investigated locus is based on Ensemble database and shown on top of the chromosome line. The exact chromosomal position of all the conserved genes is reported in the Supplementary Tables S1. The direction of the arrows indicates the gene orientation in respect to the reference gene. (B) Trimeric intracellular cation channel A (TRIC-A) and B (TRIC-B) domains are conserved among human, mouse and zebrafish. The 7 transmembrane domains (TM) are indicated. In red is shown the KEV pore channel domain, while in blue are indicated the two glycine reach regions in TM2 and TM5. (C) In situ hybridization analysis of 48 and 72 hpf WT embryos revealed the presence of tmem38b in the region of craniofacial cartilages. (D) Relative expression of tmem38a and tmem38b was evaluated by qPCR demonstrating a maternal expression only for tmem38b. The experiment was perfomed in duplicate. Scale bar: 500 µm. Human TMEM38A ENSG00000072954, murine Tmem38a ENSMUSG00000031791, zebrafish tmem38a ENSDARG00000024047; human TMEM38B ENSG00000095209, murine Tmem38b ENSMUSG00000028420, zebrafish tmem38b ENSDARG00000100549. Tric-a: human Q9H6F2, murine Q3PMT8, zebrafish Q6P2T0; Tric-b: human Q9NVV0, murine Q9DAV9, zebrafish Q7ZVP8.