Figure 2

Quantification of bacterial burden in tlr2 zebrafish larvae with M. marinum or M. avium infection. Tlr2^+/+ and tlr2^?/? embryos were infected at 28 hpf by caudal vein infection with mCherry-labeled M. marinum strain Mma20 at a dose of ~ 250 CFU, or infected with ~ 4500 CFU wasabi-labeled M. avium strain MAC 101. (A) Representative images of tlr2^+/+ and tlr2^?/? embryos infected with mCherry-labeled M. marinum strain Mma20 at 4 dpi. (B, C) Quantification of bacterial burden of tlr2^+/+ and tlr2^?/? upon Mma20 infection at 1 dpi and 4 dpi. (D) Representative images of tlr2^+/+ and tlr2^?/? embryos infected with wasabi-labeled M. avium strain MAC 101 at 4 dpi. (E, F) The bacterial burden of tlr2^+/+ and tlr2^?/? upon MAC 101 infection were quantified at 1 dpi and 4 dpi. In (B, C, and E, F) data (mean ± SD) were combined from three independent experiments. Statistical significance of differences was determined by unpaired t-test for comparison between the tlr2 mutant and wild type group. ns, non-significant; *P < 0.05, **P < 0.01. Scale bar: 50 µm. Sample size (n): 64, 78 (B), 54, 72 (C), 54, 50 (E), and 45, 45 (F).