Fig. 3

Fig. 3

Knocking down p21 by siRNA reduces S-protein-induced cell senescence. A qPCR to measure the expression of p21 in ARPE-19 cells expressing scramble siRNA or siRNA to P21 followed by treatment with S-protein for 48 h. The data represent mean ± SD, (n = 3), **p < 0.01, ***p < 0.001. B Immunoblot of p21 in ARPE-19 cells that expressed scrambled siRNA or siRNA against p21. C SA-β-Gal stain in ARPE-19 cells that were transfected with scramble or siRNA-P21 followed by treatment with S-protein for 72 h. D Quantitation of the percentage of SA-β-Gal positive cells vs total cell number per view in C. The data represent mean ± SD, (n = 3), **p < 0.01, ***p < 0.001. E SA-β-Gal stain assay, ARPE-19 cells were transfected with empty vector, Flag-S and Flag-S + siRNA-P21 for 72 h. F Quantitation of the percentage of SA-β-Gal positive cells vs total cell numbers per view in E. The data represent mean ± SD, (n = 3), ***p < 0.001