Fig. 7

(A) Pulldown of Pits::GFP^DH from fly heads indicates that endogenous CkIα interacts with Pits detected by Western blotting using anti-CSNK1E antibody (CkIα size is ~38 kDa). Interaction observed in triplicate. (B and C) Coexpression of UAS-IRF2BPL with UAS-CkIα-IR, but not UAS-LacZ, in the wing disc using nub-GAL4 at 18°C restores adult wing margin morphology (B) and Wg expression along the DV boundary (C). Scale bar, 40 μm. (D) Pits::GFP^DH; nSyb-GAL4, UAS-deGradFP coexpressing either UAS-LacZ or UAS-CkIα::HA. Time (seconds) required for flies of the indicated genotypes to climb past 7 cm (n > 22 per genotype). Statistical analyses were determined by two-tailed Student’s t test. (E) Survival curves for Pits::GFP^DH; nSyb-GAL4, UAS-deGradFP coexpressing either UAS-LacZ or UAS-CkIα::HA. n is a minimum of 49 flies per genotype (****P < 0.0001). Statistical analyses were determined by the log-rank test. (F) In vitro coimmunoprecipitation of CKIα (CSNK1A1) or GFP as a control in SH-SY5Y cells showing an interaction with endogenous IRF2BPL. IgG, immunoglobulin G. (G) SH-SY5Y cells stained with IRF2BPL and CSNK1A1. Scale bar, 10 μm. (H) Schematic depicting the canonical Wnt pathway, highlighting potential actions of Pits/IRF2BPL.