IMAGE

Fig. 7

ID
ZDB-IMAGE-230205-26
Source
Figures for Marcogliese et al., 2022
Image
Figure Caption

Fig. 7

Pits physically and genetically interacts with CkIα.

(A) Pulldown of Pits::GFPDH from fly heads indicates that endogenous CkIα interacts with Pits detected by Western blotting using anti-CSNK1E antibody (CkIα size is ~38 kDa). Interaction observed in triplicate. (B and C) Coexpression of UAS-IRF2BPL with UAS-CkIα-IR, but not UAS-LacZ, in the wing disc using nub-GAL4 at 18°C restores adult wing margin morphology (B) and Wg expression along the DV boundary (C). Scale bar, 40 μm. (D) Pits::GFPDH; nSyb-GAL4, UAS-deGradFP coexpressing either UAS-LacZ or UAS-CkIα::HA. Time (seconds) required for flies of the indicated genotypes to climb past 7 cm (n > 22 per genotype). Statistical analyses were determined by two-tailed Student’s t test. (E) Survival curves for Pits::GFPDH; nSyb-GAL4, UAS-deGradFP coexpressing either UAS-LacZ or UAS-CkIα::HA. n is a minimum of 49 flies per genotype (****P < 0.0001). Statistical analyses were determined by the log-rank test. (F) In vitro coimmunoprecipitation of CKIα (CSNK1A1) or GFP as a control in SH-SY5Y cells showing an interaction with endogenous IRF2BPL. IgG, immunoglobulin G. (G) SH-SY5Y cells stained with IRF2BPL and CSNK1A1. Scale bar, 10 μm. (H) Schematic depicting the canonical Wnt pathway, highlighting potential actions of Pits/IRF2BPL.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci Adv