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Fig. 4

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Publication: Mamot et al., 2021 - Ethylenediamine derivatives efficiently react with oxidized RNA 3' ends providing access to mono and dually labelled RNA probes for enzymatic assays and in vivo translation
All Figures: Figures for Mamot et al., 2021

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Fig. 4

Application of dually labelled RNA probes for enzymatic activity monitoring. (A) Sequence and theoretical secondary structures of Cy5-RNA₃₅-Cy3, Cy5-m⁷GRNA₃₅-Cy3 and Cy5-m⁷GRNA₂₇₆-Cy3 probes and illustration of studied DNA sequences complementary to regions of the probes. (B) Time-dependent changes of emission spectra and Cy3/Cy5 fluorescence intensity ratio after addition of RNase A, RiboLock and RNase A, RNase T1, RNase R or Dcp1/2 to Cy5-RNA₃₅-Cy3 or Cy5-m⁷GRNA₃₅-Cy3 probes. (C) Time-dependent changes of emission spectra, PAGE of reaction products, and Cy3/Cy5 fluorescence intensity ratio after addition of RNase H to probe (Cy5-RNA₃₅-Cy3 or Cy5-m⁷GRNA₂₇₆-Cy3) or DNA-probe duplex.

Acknowledgments

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