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Fig. 3. Agrp overexpression increases tumor onset in an embryo injection model of melanoma. (A) Schematic of embryo injection experiment to determine tumor onset with and without zAgRP1. Created with Biorender.com. (B,C) Representative images of male (B) and female (C) fish with tumors from empty vector (EV) control or zAgRP1 overexpression. (D) Tumor onset of MiniCoopR-rescued male and female fish combined. (E,F) Tumor onset of MiniCoopR-rescued male (E) and female (F) fish separated from D. Fish were injected at the one-cell stage and monitored for tumors at the indicated time points starting at 3 months post-fertilization (mpf). Data are the averages of three biological replicates. Log-rank (Mantel-Cox) .