FIGURE 1
RNA-Seq analysis of wildtype zebrafish with or without acrolein (ACR) treatment at larval stage. (A) Scheme of experimental design for larval RNA-Seq. 30 larvae per clutch, 6 clutches of WT and WT & ACR zebrafish larvae at 5 dpf were applied for RNA-Seq analysis. (B) Volcano plot showed significantly up-regulated (red dots) genes (logFC > 1, adj.P.val < 0.01) in WT & ACR zebrafish larvae. (C) Gene expression patterns between WT and WT & ACR zebrafish larvae at 5 dpf were analyzed using PCA. The PC1 and PC4 are represented on the X-axis and Y-axis, respectively. PCA, principal component analysis; PC1, principal component 1; PC4, principal component 4; dpf, day post fertilization.