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Fig. 9 Injected nanoparticles do not cross the BBB of zebrafish larvae. Zebrafish larvae were injected with 4 nl nanoparticles of PLGA (C,D) or PLGA-PEG (E,F), loaded with Nile Red fluorochrome. Injections were done into the posterior cardinal vein (indicated by arrows) on larvae aged 2 days post-fertilisation. Imaging was done with confocal microscopy 2 h and 1 day post-injection. Fluorescence images of larvae (A-F) are Z-projections obtained by summation of overlying pixels in the confocal Z-stack, and is shown in ?Red hot? mode, where the fluorescence signal is gradient coloured by its intensity, ranging from red (low) to white (high). Scale bars represent 500 µm.