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Fig. 4 Good shelf-life of nanocarriers is demonstrated by size stability and limited drug release. Freshly prepared batches of CPZ-loaded PLGA and PLGA-PEG were resuspended in PBS at pH 7.4 (A) or pH 9.5 (B,C), and kept at 4 °C in the dark and in sealed glass containers. Samples were taken at given time points and analysed for size (A,B) using DLS, and drug release (C) using HPLC as described in the Methods section. The release data represents the ratio of CPZ found in the pellet (nanoparticles) and supernatant (free form), after separation by centrifugation. The data show the average and standard deviation from three separate batches for each formulation.