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Fig. 2 CPZ exhibits synergistic activity with several anti-AML drugs. MOLM-13 cells were incubated for 24 h with CPZ (10 µM) alone, or with either EME (50 or 120 nM) (A) or VPA (1.0 or 1.5 mM) (B) and viability measured by metabolic conversion of the WST-1 reagent. (C): Coefficient of drug interaction (CDI) was calculated as described in the Methods section (see Eq. (5)). A CDI < 1 indicates synergism, a CDI = 1 indicates additivity, and a CDI > 1 indicates antagonism. Results are from a triplicate experiment. Asterisks *, **, and *** indicate p ? 0.05, ? 0.01, and ?0.001, respectively, from ANOVA Oneway (Bonferroni) statistical analysis (A,B) and one-sample two-sided t-test (C).