Fig. 5

Dopamine and th1 immunoreactivity in the hypothalamus of 6 dpf WT Turku zebrafish larvae. A-C, Ventral views of whole-mount 6 dpf larval brains, anterior to the left, processed for dopamine (DA) and tyrosine hydroxylase (Th1) double immunostaining. Sample fixed with a combination of glutaraldehyde and paraformaldehyde to allow visualisation of both dopamine and th1. It is noteworthy that the fixation required for double staining compromised the signal from both antibodies compared with conditions under which both antibodies perform optimally. Most dopamine-containing neurons are seen in the posterior hypothalamic group 10b, which contains very few weakly Th1-immunoreactive neurons. In more rostral neuron groups (arrowheads) both Th1 and dopamine are seen, and some neurons clearly contain both markers (small arrows). Scale bar = 75 µm