ZFIN Image: Newton et al., 2023, Fig. 1

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Figure Caption

Fig. 1

Experimental microflume used to conduct rheotaxis assays under IR illumination.

a The microflume (220 × 100 × 40 mm) with a removeable working section (30 × 30 × 10 mm) was 3D printed from translucent resin and placed on top of an infrared (850 nm) LED array. b Schematic of experimental set-up. The IR light passed through the flume and the overhead camera recorded rheotaxis trials at either 200 or 60 fps onto SD cards. The timing and duration for the camera and flume pump onset and offset of was controlled by an Arduino and pump voltage (i.e., water flow velocity = 9.74 mm s⁻¹) was controlled by a rheostat. Each trial was monitored via the live camera feed displayed on the PC and all videos were copied in duplicate onto a 12TB RAID array. Dots on fish larva indicate seven body positions tracked by DeepLabCut.

Acknowledgments

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