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FIGURE 1
Antianxiety effects of nutrient combinations. (A) Representative trace map of the zebrafish behavioral tests (n = 10). The moving speed <4, 4–20, and >20 mm/s were marked in green, black, and red lines, respectively. (B) Moving distance of zebrafish in each group (n = 10). (C) Cortisol production level of zebrafish in each group (zebrafish in three wells were combined and measured as one sample, n = 3). (D) The cortisol production level of zebrafish in each group was plotted against the distance measured in the behavioral test. n = 3. Data in panels (B–D) were presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 compared with anxiety model. In panel (B), Stachyose + PS128 (83.3 + 500 μg/ml) (#) vs. WP + Th (14.2 + 33.3 μg/ml) (#), P = 0.003. Data were collected from three biological replicates.