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Figure 1
Detection of Lasp1 protein expression in zebrafish. (A) Western blotting analysis of Gapdh and Lasp1 at different embryonic stages (24 hpf, 48 hpf, 72 hpf and 6 dpf) and in different tissues from zebrafish adults (muscle, eye, brain, liver, intestine, and gastrointestinal tract tissues). Human hepatocellular carcinoma cells, HA22T/VGH, with high levels of LASP1, were used as positive controls. (B) Localization of Lasp1 evaluated using light-sheet fluorescence microscopy in zebrafish at 72 hpf. Overview of muscle fibers and liver. Negative control is a representative overview of the “trunk muscle fibers” without primary anti-Lasp1 antibodies and with the fluorescent-labeled secondary antibodies. The images are representative of at least two independent experiments.