Figure 8

(A) Representative chromatographs are shown: lipid extractions from whole wild-type larvae (top) and whole lrpprc homozygous mutant larvae (middle). Peaks represent different lipid species and are quantified by peak area. Liver-specific rescue of lrpprc^{GBT0235/GBT0235} mutants restores nonpolar lipid levels to wild-type levels in liver-specific rescued homozygous mutant larvae (bottom). All peaks were normalized to the amount of non-metabolizable fluorescent reagent ingested (NM). (B) Excerpts from representative chromatographs, wild-type larvae (top), and lrpprc homozygous mutant larvae (bottom). Peaks or peak areas (labeled as 1–4) were individually analyzed for contribution to the overall higher NPL level in lrpprc homozygous mutants compared to their wild-type siblings. (C) 95% CI plot: lrpprc^{GBT0235/GBT0235} generated 2.04 times more non-polar lipids compared to their wild-type siblings (lrpprc^{GBT0235/GBT0235}/lrpprc^+/+=2.040, p-value = 0.019). p-Value was obtained from the standard normal z-table. (D) 95% CI plot: Tg(fabp10:Cre)lrpprc^{GBT0235/GBT0235} restored the levels of non polar lipids as compared to lrpprc^{GBT0235/GBT0235} homozygous mutants. PL = phospholipids; NPL = nonpolar lipids (triglycerides, diglycerides); CE = cholesteryl ester; NM = normalizer for amount eaten. (Figure 8—source data 1).