Figure 3

(A) Representative images of 6 dpf lrpprc^{GBT0235/GBT0235} with RFP expression in the liver and gut (magnification-5×; scale-bar: 200 µm). (B) Relative expression of lrpprc transcript in lrpprc^+/+ and homozygous mutant larvae, lrpprc^-/- (P-value = 0.0015). p-value was determined using the unpaired t-test. Each data point represents a biological replicate (N=5) (Figure 3—source data 1). (C) Mitochondrial network marked by EGFP in the caudal fin was observed to be overlapping with truncated Lrpprc:mRFP fusion protein in 2 dpf Tg(MLS:EGFP)lrpprc^GBT0235/+ embryo (scale bar: 15 µm). (D) RFP from truncated Lrpprc:mRFP fusion protein was observed to be overlapped with EGFP in mitochondria present in myocytes of skeletal muscle region in 2 dpf Tg(MLS:EGFP)lrpprc^GBT0235/+ embryo injected with NLS:TagBFP RNA. Nuclei were marked by TagBFP protein (scale-bar: 15 µm).