ZFIN Image: Gong et al., 2023, Fig. 2

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Figure Caption

Fig. 2

Activation of mTORC1 is insufficient for ? cell proliferation.

a Representative images of ? cells in control, Tg?^CA-MTOR (Tg(gcga:MTOR^L1460P, cryaa:tagRFP)), Tg?^CA-Rheb (Tg(gcga:Rheb^S16H, cryaa:tagYFP)), and gcgr^DKO (gcgra?/?;gcgra?/?) larvae at 5 dpf (scale bar, 10 ?m). b Quantification of ? cell number in control, Tg?^CA-MTOR, Tg?^CA-Rheb and gcgr^DKO larvae at 5 dpf (data represent the means � SD, n = 15 for each group). c Quantification of ? cell size in control, Tg?^CA-MTOR, Tg?^CA-Rheb and gcgr^DKO larvae at 5 dpf (Data represent means � SD, n = 16 for each group). d Representative images of EdU staining in control, Tg?^CA-MTOR and Tg?^CA-Rheb larvae at 5 dpf. EdU (red)-positive ? cells are indicated by arrows (scale bar, 10 ?m). e Quantification of EdU-positive ? cell numbers in control, Tg?^CA-MTOR and Tg?^CA-Rheb larvae at 5 dpf (data represent the means � SD, n = 9 for each group). ***P < 0.001, ****P < 0.0001, NS indicates no significant difference (one-way ANOVA, Tukey?s multiple comparisons test, the quantifications represent individual islet sections). Source data are provided as a ^{Source Data} file.

Acknowledgments

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