ZFIN Image: Yates et al., 2021, Fig. 6

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Figure Caption

Fig. 6

High-throughput sequencing of a SLALOM-generated sgRNA library to normalized cDNA extracted from 48 hpf zebrafish hearts. (A) Schematic diagram of the library generation process. B) Results of qPCR analysis on five genes with varying expression levels in the endogenous tissue before and after cDNA normalization. (C) Histogram showing the distribution of spacer lengths within the library. (D) Venn diagram of the genes targeted by at least one spacer in the library compared to genes detected in the 48 hpf zebrafish heart by RNA-seq. A threshold of 1 read per gene was used to designate both genes and spacers as ‘detected’. (E) Comparison of the total number of spacer reads vs. expression level in an RNA-seq dataset for 48 hpf zebrafish hearts.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.