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Fig. 4
The effect of the protein tyrosine phosphatase receptor-ζ1 (PTPRZ1)-selective tyrosine phosphatase inhibitor MY10 in zebrafish heart. A: MY10 was added in the water of 24 hpf zebrafish embryos and the latter were observed under a stereoscope and photographed at 72 hpf. Scale bar corresponds to 100 μm. B: HR is expressed as means ± SD of heart beats/min (DMSO 0.1%: n = 9, MY10 10−5 M: n = 10). C: MY10 was injected at 1-cell stage embryos and the injected embryos were observed and photographed at 72 hpf. Scale bar corresponds to 100 μm. D–H: quantification of cardiac function of injected embryos at 5 dpf. Data are expressed as means ± SD (DMSO: n = 6, MY10: n = 8). Statistical analysis was performed by Student’s unpaired t test. dpf, days postfertilization; EDV, end-diastole volume; ESV, end-systolic volume; hpf, hours postfertilization; HR, heart rate; %EF, percent ejection fraction; SV, stroke volume.