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Figure 7 Inhibition of Fut8 function by 2FF inhibits myotube formation (a) Changes in muscle differentiation after the addition of 0, 0.5, and 1 mM 2FF. Scale bar: 100 μm (b) Immunostaining of C2C12 cells on day 5 of induced muscle differentiation using anti-MY32. Scale bar: 100 μm. (c) Diameter of myotube (one-way ANOVA, p < 0.01, n = 3). (d) Length of myotube (one-way ANOVA, p < 0.01, n = 3). (e) Histogram of fusion index (one-way ANOVA, p < 0.05). (f) Differentiation index (one-way ANOVA, p < 0.05). (g–j) Quantitative RT-PCR using the delta-delta Ct method for Myomaker, Myomerger, Ckm, and Myogenin expression during differentiation of C2C12 cells. Relative control normalized by r18 and Day 0 (n = 3).