ZFIN Image: Chen et al., 2022, Figure 6

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Figure 6

Anti-biofilm activity of Sp-LECin against P. aeruginosa. (A) The inhibitory effect of Sp-LECin on the formation of P. aeruginosa biofilm. The biofilm mass was quantified by crystal violet (CV) and the absorbance at 595 nm was measured. (B) The inhibitory effect of Sp-LECin against the preformed biofilm of P. aeruginosa. The amount of reduced resazurin and the residual amount of oxidized resazurin was determined by measuring at the absorbance at 560 nm and 620 nm, respectively. The corrected A560 value (AR560) was calculated using the following formula: AR560 = A560 ? (A620 � RO) and RO = AO560/AO620, where A560 and A620 are sample absorbance and AO560 and AO620 are the absorbance of medium containing 0.1 mM resazurin. Significant difference between control group and Sp-LECin treatment group was indicated by asterisks as * p < 0.05, ** p < 0.01 and *** p < 0.001. The bars indicate the mean � standard error of mean (n = 5).

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