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Figure 5
Effect of Sp-LECin on ROS production in P. aeruginosa. Intracellular ROS was monitored using 2,7-dichlorofuorescin diacetate (DCFH-DA) probe after treatment with different concentrations of Sp-LECin (0, 12, 24 and 48 ?M) for 0.5 (A) and 1 h (B), respectively. LL-37 was used as a positive control. Significant difference between control group and AMP treatment group was indicated by asterisks as *** p < 0.001. The bars indicate the mean ± standard error of mean (n = 5).
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