ZFIN Image: Jim et al., 2022, Figure 4

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Figure Caption

Figure 4

Cxcl18 expression in zebrafish larvae infected with pneumolysin-positive or pneumolysin-negative S. pneumoniae D39V

(A) Top 20 highest significantly enriched genes in zebrafish infected with S. pneumoniae PLY+ or S. pneumoniae PLY− (q < 0.05).

(B) Gene expression analysis of cxcl18b during early stages of pneumococcal meningitis. RT-qPCR was performed on pools of 20 zebrafish larvae injected with 2,000 CFUs of S. pneumoniae PLY+, S. pneumoniae PLY−, or control PBS injection and collected at 3, 6, and 9 hpi. The data represent four biological replicates; F(1, 6) = 44.21, ^∗∗adjusted p = 0.0006, PLY+ versus CTRL versus PLY− versus CTRL; determined by two-way ANOVA on log2-transformed values; ^∗∗p = 0.0063; determined by two-way ANOVA with Tukey’s post test on log2-transformed values.

(C) Expression of Cxcl18b:EGFP is higher in zebrafish larvae (2 dpf) injected with 2,000 CFUs of S. pneumoniae PLY+ compared with infection with similar CFUs of S. pneumoniae PLY− or control PBS injection. Scale bar: 500 μm.

(D) Quantification of Cxcl18b:EGFP expression in the head region over time. The data represent the mean ± SEM with 10 larvae per group; ^∗∗∗∗p < 0.0001; determined by two-way ANOVA followed by Tukey’s post hoc test.

Acknowledgments

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