FIGURE 8

FIGURE 8

Glycolysis inhibition in regeneration. (A) Schematic of the experimental design. Embryos were amputated and incubated in treatment solution for 72 h or 120 h as indicated by the blue (oxamate) and green (2DG) arrows. Black asterisks indicates time point for imaging, at 5 dpa upon completion of regeneration. (B) Micrographs of representative Tg[ubb:laconic]^lkc1 larvae tails, pseudocoloured to show Laconic ratio. Imaged at 7 dpf or 120 hpa when amputated at 48 hpf, treated for the first 72 h post amputation with 25 mM 2DG, for the full 120 h of regeneration with 10 mM oxamate, or with a control. (C) Graph showing quantification of raw Laconic ratios at 7 dpf or 120 hpa when amputated at 48 hpf and treated for the first 72 h post amputation with 25 mM 2DG, 10 mM oxamate, or control. Two‐way ANOVA to calculate significance, n = 13. White dashed box in inset (i) shows example of area measured for quantification of Laconic ratio in (C). (D) Brightfield images of representative Tg[ubb:laconic]^lkc1 larvae tails at 7 dpf or 5 dpa when amputated at 48 hpf, treated for the first 72 h post amputation with 25 mM 2DG, for the full 120 h of regeneration with 10 mM oxamate, or with a control. Red dashed line indicates measurement taken for fin length. (E) Graph showing fin length measurements taken at 7 dpf or 120 hpa when amputated at 48 hpf and treated for the first 72 h post amputation with 25 mM 2DG or with a vehicle control. Two‐way ANOVA to calculate significance, n = 13. All scale bars represent 200 μm. Differences were considered significant to * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns P ≥ 0.05.