Lactate levels in tail regeneration. (A) Micrographs of representative Tg[ubb:laconic]lkc1 embryos tails at 48 hpf imaged pre‐amputation and at various time‐points over the course of regeneration (note that images show representative embryos rather than following an individual embryo), pseudocoloured to show Laconic ratio. (B) Graphs showing quantification of raw Laconic ratios pre‐treatment and at various timepoints post amputation. Two‐way ANOVA to calculate significance, n = 24. (C) Graph showing quantification of raw Laconic ratios specifically in the notochord bead region (as indicated in (i)). Students' t‐test to calculate significance, n = 24. Scale bar represents 200 μm. Differences were considered significant to * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns P ≥ 0.05.
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