FIGURE 3

FIGURE 3

Lactate levels in fin fold regeneration. A) Micrographs of representative Tg[ubb:laconic]^lkc1 embryos tails at 48 hpf imaged pre‐amputation and the same individual embryo followed over the course of one‐hour post amputation, pseudocoloured to show Laconic ratio. (B) Micrographs of representative transgenic Tg[ubb:laconic]^lkc1 embryos tails amputated at 48 hpf and imaged at given time‐points over the course of regeneration until five days post amputation, pseudocoloured to show Laconic ratio. (C) Graph showing quantification of raw Laconic ratios pre‐treatment and over the course of one‐hour post amputation. Two‐way ANOVA to calculate significance, n = 18. (D) Graph showing fold change between pre‐amputation ratio and 10 min post amputation. Students' t‐test to calculate significance, n = 56. (E) Graphs showing quantification of raw Laconic ratios pre‐treatment and at various timepoints post amputation. Two‐way ANOVA to calculate significance, n = 18. All scale bars represent 200 μm. Differences were considered significant to * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns P ≥ 0.05.