FIGURE 6

FIGURE 6

Mitochondrial inhibition in wound healing. (A) Schematic of the experimental design. Embryos were amputated in the treatment solution and incubated for one‐hour before washing out the drug, and maintained until regeneration was complete at 120 hpa. Green arrow indicates period of sodium azide (NaN₃) treatment and black asterisks indicate time points for imaging. (B) Micrographs of representative Tg[ubb:laconic]^lkc1 embryos tails treated at 48 hpf for one‐hour post amputation with 15 mM NaN₃, to induce prolonged glycolysis and lactate production, or PBS control. Images were acquired at pre‐amputation, 10 min, one‐hour, and five‐days post amputation, and pseudocoloured to show Laconic ratio. (C) Graph showing quantification of raw Laconic ratios pre‐, 10 min post‐, and five‐days post‐amputation. Two‐way ANOVA to calculate significance, n = 12. (D) Graph showing measured fin widths/length in micrometres pre‐, 10 min post‐, and five‐days post‐amputation. Fin length measured from the tip of the notochord to the distal edge of the fin fold. Two‐way ANOVA to calculate significance, n = 12. All scale bars represent 200 μm. Differences were considered significant to * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns P ≥ 0.05.