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Figure 3 setd5 haploinsufficiency causes a growth delay in adult mutant zebrafish. (a) Representative images of 10-month-old setd5+/+ and setd5+/− zebrafish adults. Scale bar 500 μm. (b) Body length and body weight analysis in setd5+/+ and setd5+/− 10 months-old zebrafish adults. n = number of adults analyzed. (c) Representative images of adult brains from 10-month-old setd5+/+ zebrafish adults, evidencing the different measurements conduced for the morphometrical analysis included in (d). W, width; L, length. (d) Relative Telencephalon (Tel) length and width, Optic tectum (TeO) length and width, Cerebellum (Ce) length and width, Diencephalon (Die) length and width in adult brain, normalized to the entire brain length, from 10-month-old setd5+/+ zebrafish adults. Absolute values are included in Figure S5. (d) n = number of adult brains analyzed. Data are expressed as mean ± SEM. Statistical analysis was performed by Student’s t-test. * p < 0.05; ** p < 0.01.