Figure 5.
(A) Schematic of larval zebrafish whole-cell paired recording. (B) Simultaneous current-clamp and voltage-clamp recording of sister V2a/b neurons. Current step-evoked spiking in sister V2a neuron and simultaneous voltage-clamp recording in V2b (left). Current step-evoked spiking in sister V2b neuron and simultaneous voltage-clamp recording in V2a (right). No synaptic responses are seen in either case. (C) Bar graph showing the number of clonal V2a/b interconnected pairs detected (n = 10 pairs from 10 fish). (D) Bar graph showing the number of V2a/b interconnected pairs detected for non-sister pairs (n = 7 pairs from seven fish).
V2a/b sister neurons do not synapse with each other.
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