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Figure 1
(A) Schematic of vsx1 GFP+ progenitor undergoing a final paired division into sister V2a/b neurons. (B) Schematic of fertilized embryo injection and screening for vsx1 GFP+ progenitors at the 21-somite stage. (C) Time-lapse single-plane confocal images taken every 5 min as a vsx1 GFP+ progenitor divides into two sister neurons, imaged at 24 hr post fertilization (hpf). (D) Confocal imaging of vsx1+ sister neuron pairs in the spinal cord of 4 dpf larvae. Left: vsx1 GFP+ sister pair in a chx10:Red larva. One sister neuron is co-labeled (white, V2a) while the other is a presumed V2b. Middle: vsx1 GFP+ sister pair in a gata3:Red larva showing an identified V2b with a presumed V2a or V2s. Right: vsx1 mCherry+ sister pair in a sox1:GFP larva, showing an identified V2s with a presumed V2b. Colors switched for label and image consistency. (E) Bar graph displaying the fraction of vsx1 GFP+ pairs in chx10:Red (n = 92), gata3:Red (n = 38), and sox1:GFP (n = 65) larvae in which either 0/2 sister neurons were co-labeled with the reporter (black), 1/2 sister neurons were co-labeled (green), or 2/2 sister neurons were co-labeled (gray). Source data for this figure are given in Figure 1—source data 1.
Sparse vsx1+ progenitor labeling allows for clonal pair tracking in vivo.