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Fig. 11
Immunofluorescence staining of Tnf-α on cryo-sectioned embryonic tissues. GFP-positive myocardium (A–C) and Alexa Fluor 568 dye which indicates Tnf-
(A′–C′) are displayed. Relative fluorescence intensity plot of heart region (D) indicates that Tnf-α was significantly up-regulated in treated hearts (n = 5 for each group). Mean and standard deviations are shown, and a two-sample t-test was used, where *P < 0.05. Scale bars: 50 μm
(A′–C′) are displayed. Relative fluorescence intensity plot of heart region (D) indicates that Tnf-α was significantly up-regulated in treated hearts (n = 5 for each group). Mean and standard deviations are shown, and a two-sample t-test was used, where *P < 0.05. Scale bars: 50 μm
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