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Fig. 5 (A) LSFMM image and spatial WSS contour of 5 dpf treated heart I (pre-washout) during systole and diastole, respectively. A, atrium; V, ventricle. Spatial–temporal WSS assessment of controls at 5 dpf (hearts I, II, and III), and treated hearts at 5 dpf before and after BDM washout. Treated hearts I and II were collected before BDM washout and treated hearts III and IV were analyzed after BDM removal. Area-averaged WSS experienced at the inlet, mid-ventricular, and outlet were measured over a course of cardiac cycle for controls and treated hearts (B–G), respectively. (H) Time-averaged WSS (TAWSS) of controls and treated hearts. (I) Oscillatory shear index (OSI) of treated hearts I, II, III, and IV at inlet and outlet regions. Note that OSI of controls was nearly zero based on the calculated oscillatory velocity index