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Fig. 4 Controls (n = 5) and treated hearts before (n = 5) and after (n = 5) washout were quantified for (A–C) ventricular outlet velocity, (D) heart rate, (E) ejected stroke volume, and (F) ejected cardiac output. For (D–F), mean and standard deviations were presented and a two-sample t-test was used, where *P < 0.05. Note that the heart rate plot (D) differs from Figure 3B as the chosen embryos were specifically assessed for other parameters and the sample size was limited to five
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