Figure 2

Figure 2

RT-qPCR and transcriptome data analysis of the DFNA58 genes and the reference/control genes. (A,B) Comparison of the level of expression of each gene in each tissue using 1/deltaCt values (RT-qPCR). (C–G) Relative quantification (delta-delta Ct of Pfafll [45]) regarding each DFNA58 gene (other genes in the Supplementary Figure S1) in different tissues of the adult zebrafish (lagena + utricle as the reference sample, the other tissues being fold of its expression). In (H–L), results of the reanalysis of data from transcriptomes, comparing the expression levels of genes from the DFNA58 locus and the reference genes regarding the zebrafish inner ear hair cells (HC) and their nonsensory surrounding cells (nsSC) in (H) and regarding the DFNA58 genes in the microglia [35], liver [34], and inner ear cells [25], as made available for public access by authors for reanalysis as part of open science. LG + UT: lagena + utricle; BRN: brain; HRT: heart; SWB: swim bladder; IE: inner ear. *,**,***,**** mean increasing levels of significance.