ZFIN Image: Lei et al., 2021, Fig. 2

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Figure Caption

Fig. 2

par1 is required for lymphatic differentiation in zebrafish embryos

(A) Immunostaining of prospero homeobox protein-1a (Prox1a) in Tg(fli1a:EGFP) siblings and par1 homozygous mutants at 54 hpf. White arrowheads indicate the presence of parachordal lymphangioblasts (PLs) sprouting in each somite; blue arrowheads with white dashed lines represent positive Prox1a staining in PLs. Scale bars: 100 μm.

(B) Prox1a-positive PLs in siblings (n = 17 embyos) and par1 homozygous mutants (n = 22 embryos) at 54 hpf; 7 somites/embryos were used for quantification. Table shows the number of prox1a-positive segment per 7 somites in each embryo.

(C) Relative mRNA expression of PROX1 and PAR1 in human dermal lymphatic endothelial cells (HDLECs) after ctr-siRNA (control) and PAR1-siRNA transfection.

(D) Western blot analysis of PROX1 and PAR1 expression upon PAR1 knockdown in HDLECs. In (C and D), values represent means ± SEMs. ∗p ≤ 0.01, ∗∗p ≤ 0.001 in the Student's t test.

Acknowledgments

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