ZFIN Image: Yin et al., 2021, Fig. 2

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Figure Caption

Fig. 2

FIGURE Identification of F2 fbn1 ^+/? zebrafish. (a) PCR amplification of the genomic DNA from TU wild?type and F1 fbn1 ^+/? zebrafish. M, DNA marker. (b?d) PCR amplification of the genomic DNA of F2 fbn1 ^+/? adults. Lanes 9 and 26, DNA Marker; lanes 3, 4, 6, 8, 12?14, 17, 18?20, 22, 25, 27, 29?32, and 34 in (b), heterozygous for alleles A and B mutants; lanes 1, 2, 5, 7, 9?11, 15, 16, 21, 23, 24, 26, 28, and 33 in (b), wild?type; lanes 1?3, 10?12, 14?16 in (c), heterozygous mutants of alleles A and B; lanes 4?9, 13, and 17 in (c), wild?type; lanes 1, 3, 4, and 7 in (D), heterozygous mutants of alleles A and B; lanes 2, 5, 6, 8, and 9 in (d), wild?type. (e) DNA sequencing results of F2 fbn1^+/? fish. Red arrow indicates the position of the mutation

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