ZFIN Image: Wang et al., 2022, Figure 8

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Figure 8

Reconstitution of Smyd3 in Smyd3-deficient cells promotes hypoxia-induced apoptosis. A, apoptotic cells in Smyd3-deficient MEF cells reconstituted with or without wildtype Smyd3 by lentivirus under normoxia or hypoxia detected by flow cytometry analysis. Data show mean ± SD; Student’s two-tailed t test. ∗∗∗p < 0.001. Data from three independent experiments. B, Smyd3-deficient MEF cells were reconstituted with or without wildtype Smyd3 by lentivirus and treated with DFX (150 μM) or DMSO as a control for 24 h. Apoptotic cells were detected by fluorescence microscopy. Scale bar = 100 μm. DFX, deferoxamine mesylate salt; MEF, mouse embryonic fibroblast.

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