Figure 9

The effect of deleting the sapS gene in S. aureus on the transcription of genes that code for enzymes involved in the detoxification of reactive oxygen species (ROS). qRT-PCR was used to conduct quantitative transcript analyses of ahpC (A), katA (B), crtM (C), sodA (D) and sodM (E) in SA564 (black bars) and SA564 ?sapS (blue bars) cells cultured in TSB at 37 °C and 225 rpm at the time points indicated. Quantification of the transcripts was performed in relation to the transcription of gyrase B (in copies per copy of gyrB). The data are shown as mean + SD of five biological replicates. ns, not significant; * p < 0.05 (Mann-Whitney U test between WT and mutant at a given time point).