Figure 6

MPO, KC, and TNF-? contents in kidney tissues obtained from mice at day 4 post infection. C57BL/6N mice were infected with S. aureus strain SA564 (black symbols), SA564 ?sapS (blue symbols), and the cis-complemented derivative SA564 ?sapS::sapS (grey symbols), respectively (n = 10 per group). Four days after infection, mice were euthanised, kidneys were extracted and blended with PBS solution, the tissue homogenates centrifuged, and the MPO (A), KC (B), and TNF-? (C) contents in the supernatants determined by enzyme-linked immunosorbent assays. Kidney homogenates from age-matched uninfected mice served as control (white symbols). Each symbol represents the amount of enzyme/cytokine found in the kidney homogenate of an individual mouse. The median of all the observations is shown by the horizontal bar. ns, not significant; * p < 0.05; **, p < 0.01 (Kruskal?Wallis test followed by Dunn?s post hoc test. Only significant differences between WT-infected and mutant-infected mice are shown).