ZFIN Image: Ahmad-Mansour et al., 2022, Figure 4

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Figure 4

The virulence of S. aureus SA564 in a murine abscess model is enhanced by the presence of SapS. C57BL/6N mice were infected via retroorbital injection with 1 x 10⁷ cells of S. aureus strain SA564 (black symbols), SA564 ?sapS (blue symbols), and the cis-complemented derivative SA564 ?sapS::sapS (grey symbols), respectively (n = 10 per group). Mice were euthanised and to evaluate the bacterial burdens in the kidney (A) and liver (B) organs, livers and kidneys were excised and homogenised in PBS 4 days after infection. Serial dilutions of the homogenates were then plated on sheep blood agar plates. One mouse is represented by each symbol. The median of all observations is indicated by the horizontal bars. ns, not significant; ** p < 0.01 (Kruskal?Wallis test followed by Dunn?s post hoc test). Only differences that are statistically significant between WT and mutants are displayed.

Acknowledgments

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