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Fig. 1 Schematic overview of the LSFM platform for in vivo SMM imaging. (A) schematic of the optical illumination (top two) and detection (bottom one) light paths; (B) schematic of the sample mounting system, with (C) its lateral section view, (D) the FEP support for zebrafish mounting. L = lens, PH = pinhole, CL = cylindrical lens, GM = galvo-mirror, BFP = back focal plane, TEL = telescope plane-conjugating GM to BFP, IO = Illumination objective, DO = detection objective, BF = bandpass filter, TL = tube lens, ZF = zebrafish, RC = rotation capillary, FS = FEP support, RM = rotational motor, IC = imaging chamber, ZM = motorized stage in z, XYM = motorized stage in xy, lmpa = low melting point agarose.