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Fig. 7
D-2-HG exposure caused liver degeneration in zebrafish.
A Experimental scheme depicting the treatment of 5 dpf larvae with different concentrations of D-2-HG (0, 0.5, 1, 5, 10, and 20 mM) for 16 h before analysis. B The whole morphology of larvae. C Livers of 5-dpf larvae were performed with hematoxylin-eosin (H&E) staining and immunostained with anti-Annexin A4 and HNF-4? fluorescence intensity. D Quantification of Annexin A4 and HNF-4? in the panel (C). *P < 0.05, **P < 0.01.
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